Imageamento in Vivo por Bioluminescência e Fluorescência

MOIS HT – In Vivo Bioluminescence and Fluorescence Imaging

Name: MOIS HT - In Vivo Bioluminescence and Fluorescence Imaging
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In vivo bioluminescence and fluorescence imaging system, enabling the analysis of signals in tissues and organisms both in vivo and in vitro.

In vivo Imaging for Bioluminescence and Fluorescence

The MOIS HT from RWD is an advanced imaging system designed for in vivo and in vitro analysis of bioluminescent and fluorescent signals in tissues and organisms, supporting research applications such as tumor growth monitoring and stem cell/immune cell tracking.

Featuring a high-sensitivity CCD with 90% quantum efficiency and -90°C cooling, the system delivers exceptional performance in bioluminescence imaging. Equipped with an optimized macro camera and a range of professional imaging filters, the MOIS HT enables quick, intuitive acquisition of high-quality images and videos in real time.

With 26 narrow-band filters and advanced spectral unmixing technology, this imager enhances the accuracy and resolution of fluorescence imaging, covering a broad spectrum of fluorescent dyes used in biological research. Dedicated software (included) simplifies luminescent image analysis.

The MOIS HT combines a simple, user-friendly design with speed and reliability, making it an efficient solution for researchers.

MOIS HT - Image Acquisition Process

MOIS HT - 5-channel anesthesia tray and active scavenging masks

Overview

The MOIS HT Small Animal In Vivo Imaging System is a non-invasive imaging platform designed for real-time monitoring and visualization of biological processes in live small animals, such as mice and rats. Supporting both bioluminescence and fluorescence imaging modes, this system enables researchers to track molecular and cellular activities in living organisms—providing critical insights into disease progression, treatment efficacy, and fundamental biological mechanisms—without requiring animal euthanasia.

Main Applications of MOIS HT

Bioluminescence/fluorescence imaging in vivo and in vitro;
Animal/plant gene expression, developmental biology;
Immunity research and drug development;
Monitoring tumor development/tracking cell migration;
Molecular biology and nanoparticles.
Neuroscience

Key Features of MOIS HT

High precision

Using a scientific CCD camera with back illumination and -90°C deep cooling to effectively reduce background noise.
CCD camera with resolution >1024X1024, quantum efficiency >90%, special chip coating provides high sensitivity in the range of 500-700 nm (up to 900 nm for wider application with standard dyes).
A fixed focus lens (F/Stop≤ 0.9) collects more photons per unit of time, capturing finer details and reducing noise.
The standard equipment includes 19 excitation filters and 7 narrow-band emission filters, providing a clean signal to avoid cross-color interference and produce realistic data.
Heated bed for animals (20-42°C);
The uniformity of excitation light intensity ensures constant power across different fields of view, increasing the reliability of fluorescence data collection.
The instrument is calibrated to NIST standards for absolute bioluminescence quantification, ensuring consistent results across all imaging settings.

Excellent quantitative result

The imaging/analysis software has several ROI contour selection modes for quantitative signal analysis.
The imaging software includes an image acquisition and data analysis module that can generate a time series of multichannel bioluminescence and fluorescence imaging images as a multi-mode sequential imaging.
The imaging software has a real-time overexposure warning function.
The offline analysis software can be installed an unlimited number of times.
The number of photons emitted per unit time, per unit area, and per unit radian is taken as a quantitative unit according to an internationally recognized quantitative standard.
The system is equipped with an indicator laser to display the center of the visual field in real time.

Extensibility

Possibility of upgrading with an X-ray module.

Multifunctionality

Using four channels, blue, green, red and near infrared, most fluorescent proteins and fluorescent materials from GFP to ICG can be visualized. Since more than one fluorescent substance can be imaged, different features can be observed in a single sample. For example, tumor imaging and drug imaging can be performed on the same animal, so that targeting and tumor formation can be observed simultaneously. Bright images can also be combined to localize fluorescence within the animal.

Application of the MOIS HT

Monitoring the development of tumors and infections

Stable GFP cell lines can be used to confirm tumor development. The generated GFP-stable cell lines can be visualized in vitro using MOIS HT. GFP cells are injected into the subcutaneous tissue and fluorescent images are generated as the cells proliferate. In this way, not only can tumor size be quantified and compared, but also images of metastases to other tissues can be obtained. The intensity of the fluorescent signal changes over time, and the camera exposure time changes accordingly. The analysis software can quantify these changes by taking into account different conditions such as exposure time and gain; the results of samples with different images can also be compared and analyzed.

Tracking cell migration

Stem or immune cells with expanded functions for various purposes can be visualized in animals to determine their location and viability. Stem and immune cells are difficult to label with fluorescent genes. Therefore, the cells can be stained with fluorescent reagents in various ways. Stem and immune cells stained with fluorescent reagents can be administered to animals by various methods, such as intravenous administration, intraperitoneal administration, subcutaneous administration, etc. MOIS HT imaging can be used to determine the location of these cells. Quantitative analysis can be used to determine the viability of the cells.

Plant visualization

Plant imaging with MOIS HT allows for the acquisition of GFP-labeled plant leaves. It is difficult to image plant leaves because chlorophyll has strong autofluorescence. It is possible to remove chlorophyll autofluorescence using special filters and analyze it using GFP. The autofluorescence of chlorophyll itself can also be used as data. The degree of chlorophyll activity can be determined from the autofluorescence intensity. In addition, images can be obtained from plant seeds and callus. Fluorescence imaging can be performed throughout the entire life cycle of the plant

Targeting the biodistribution of drugs, molecules and nanoparticles

Drugs obtained in vitro can be administered to animals for experimental purposes. By taking pictures at certain time intervals, the movement and accumulation patterns of the drug in living animal tissues can be studied. Images of drugs confirmed in vivo can be re-examined in vitro. Since fluorescence persists even after the death of the animal, each tissue can be evaluated individually. The obtained in vitro data, together with the in vivo data, can serve as excellent confirmation of the experiment.

Examples of in vivo animal imaging

a. GFP -expressing tumors derived from stable cell lines after subcutaneous injection.
b. Use of GFP and ICG for fluorescence imaging.
c. Use of iRFP gene for NIR tumor imaging.
d. Migration of DiD-labeled immune cells from the tail vein to the inner part of the spinal canal.
e. Accumulation of LCG-labeled drug in the lung.
f. Migration of Cy7-labeled drug to the liver.
g. Study of the biological light signal of tumor in mice

Examples of visualization of fluorescence of different objects of study

a. Fluorescence labeling of zebrafish.
b. Visualization of cells with GFP in a 24-well plate.
c. Testing of fluorescent markers.
d. In vitro drug distribution studies.
e. GFP signal for gene expression of plant leaf pathogens using viral vectors.
f. Chlorophyll fluorescence.
g. Expression of marker genes on plant leaves.
h. Isolation of seeds with transfected genome using GFP visualization.

Technical Parameters

Imaging Modes
Fluorescence Imaging Module	√
Bioluminescence Imaging Module	√
Spectral Separation Imaging	√
X-Ray Module	Extendable upgrade
Upconversion Fluorescence Imaging Module	Extendable upgrade

Detector
Camera Type	Back-thinned, scientific-grade CCD
Camera Operating Temperature	-90℃ absolute cooling
Pixel Dimensions	1024×1024
Lens Aperture	F/stop≤ 0.95
Minimized Field of View (minFOV )	2.5cm×2.5cm
Maximized Field of View (maxFOV)	25cm×25cm
Quantum Efficiency	≥90%（500-700nm）

Laser and Related Components
Light Source	150W halogen lamp
Indicator Laser	Real-time view for center pos assist

Filters
Excitation Filters	19 filters
Emission Filters	7 filters

Software
Online Acquisition System	• Supports over - exposure alerts. • Has built-in time-series,multi-channel,multi-modefeatures. • Allows synchronous data acquisition & analysis.
Offline Analysis Workstation	• Supports offline analysis with a built-in module. • No limit on offline analysis software installations.

Environmental Control
Animal Chamber	Standard 5 - channel, optional upgrade to 10 - channe
Temperature Module	20 - 40℃ ( ±0.1℃)
Drug Administration Module	•Enable in - experiment drug addition • Support auto multi-point dosing
Anesthesia System	• Anesthetic: Isoflurane • Conc. 0 - 5% adjustable

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